Storage of erythrocytes at temperatures -20 to -24 degrees C
Daszyński, J.; Rudowski, W.; Gaczkowski, A.; Lukasiak, S.
Acta Medica Polona 22(2): 151-160
1981
ISSN/ISBN: 0001-608X PMID: 7315511 Document Number: 174974
Human erythrocytes frozen at -20 to -24.degree. C in the presence of cryoprotective solutions were evaluated in vitro. The erythrocytes were frozen in 5 different cryoprotective solutions differing in the concentration of glycerol (from 6.2 to 8.5 mol/l). Two of these solutions contained active metabolites such as adenine, inosine and sodium ascorbate. After thawing glycerol was washed out from the erythrocytes using in definite proportion sodium chloride solutions in decreasing concentrations. In vitro investigations of the erythrocytes included determinations of ATP, 2,3-DPG [2,3-diphosphoglycerate], intracellular K and erythrocyte loss in percent after thawing. The investigations were carried out at intervals of 3 mo. up to 12 mo. The evaluation of erythrocytes frozen in different cryoprotective solutions shoved that the concentration of glycerol was without any great significance for maintenance of their biological activity. The cryoprotective solution enriched with adenine, inosine and phosphate (no. IV) was found to be most useful. The concentrations of ATP and 2,3-DPG in the eythrocytes after 12 mo. of storage in this solution was unchanged and remained at the level of the initial values. The recovery of erythrocytes in vitro was 81.3%.