Polypeptides of the virus-like particles isolated from the plasma of rats with Shvec erythromyelosis
Sherban, S.D.
Ukrains'kyi Biokhimichnyi Zhurnal 48(3): 287-291
1976
ISSN/ISBN: 0041-610X PMID: 1066868 Document Number: 107162
The structural proteins of virus-like particles isolated from ascitic fluid of rats with experimental Shvec erythroleukosis were studied by polyacrylamide gel electrophoresis. The bulk of protein (60-70%) is divided into five major bands corresponding to a molecular weight of 15,000, 20,000, 34,000, 48,000 and 61,000 daltons. Besides these bands, some distinct minor ones corresponding to a molecular weight ranging from 41,000 to 82,000 daltons were always present. There were also some slight bands that are not developed in scanning on the densitometer. These bands occurred in the region corresponding to a molecular weight above 50,000 daltons. The gels stained to detect polysaccharides revealed a distinct red colour band of a molecular weight 80,000 daltons and 3 slight of a lower molecular weight which are probably the degradating products of the main glycoprotein component. The electrophoretic pattern of the 1.16 g/cm3 fraction obtained from the healthy rat liver and Ehrlich ascitic fluid differs from the electrophoregram of the virus-like particles isolated from the rats with Shvec erythroleukosis.