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Reproducibility of yields of foot--and--mouth disease virus from BHK monolayer and suspension cells

Spier, R.E.

Developments in Biological Standardization 35: 73-78

1976

ISSN/ISBN: 0301-5149
PMID: 198316
Document Number: 97829
The production of FMD virus from BHK 21 C13 monolayer and suspension cells was examined under standardized conditions and in different systems. The yields of virus from suspension cells did not significantly exceed the yield from monolayer cells, whereas the monolayer cell was capable of producing virus from some strains of FMD virus which would not grow in suspension cells. When different production systems were examined, the scale of operation did not significantly influence the yield of virus from suspension cells, while the plastic spiral film propagator produced significantly less virus than the other monolayer systems examined. The use of unit monolayer systems on the large scale is discussed.

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