Colorimetric determination of piperazine
Sasi, P.K.; Raj, R.K.
Indian Journal of Physiology and Pharmacology 20(3): 180-182
1976
ISSN/ISBN: 0019-5499 PMID: 977089 Document Number: 96869
A sensitive method for the quantitative determination of piperazine is described. The method is precise and responds linearly from 25 g to 500 mug and above of the material. The procedure is based on the formation of a complex of piperazine with reineckate in neutral or acid medium. The complex can be separated by centrifugation. It is then dissolved in acetone and estimated at 530 nm in a colorimeter. Piperazine present in trichloroacetic acid extractrs of biological samples can also be estimated by this method.