Colorimetric method for determination of ethanol in presence of methanol
Sane, R.T.; Kamat, S.S.; Pandit, A.D.
Journal - Association of Official Analytical Chemists 64(5): 1145-1148
1981
ISSN/ISBN: 0004-5756 PMID: 7287611 Document Number: 176462
A method is described for the determination of ethanol in the presence of methanol without pre-separation. The sample is oxidized to give acetaldehyde and formaldehyde. 2-Thiobarbituric acid reagent is added and selectively forms a pink adduct with acetaldehyde, but not with formaldehyde. The method is sensitive, obeys Beer's law in the concentration range 18-80 microgram/mL, and has an advantage over other methods because it does not detect any "apparent normal value" of alcohol in blood.