Chemiluminescence of the blood serum in the presence of divalent iron salts
Vladimirov, I.A.; Farkhutdinov, R.R.; Molodenkov, M.N.
Voprosy Meditsinskoi Khimii 22(2): 216-223
1976
ISSN/ISBN: 0042-8809 PMID: 16392 Document Number: 96852
On incubation of diluted blood serum with ferrous ions kinetics of chemiluminescence, typical for lipid containing systems, was observed. It comprised several sequental steps: quick flash, inhibition of the chemiluminescence, slow flash with subsequent steady-state luminescence. At each step of the phenomenon a correlation was found between the intensity of chemiluminescence, the rate of accumulation of products of lipid peroxidation (malone dialdehyde) and concentration of Fe2+ in the mixture. The data obtained suggest that chemiluminescence of blood serum in presence of Fe2+ is due to lipid peroxidation in the serum. Kinetics of the reaction and shape of the chemiluminiscence curve depended upon a number of factors: dilution of blood serum, concentration of Fe2+ added, temperature, pH and (if blood plasma was used) type of an anticoagulant use. Only in blood lipoproteins, mainly in beta-lipoproteins, the distinctive pattern of peroxidation was developed with simultaneous accumulation of malone dialdehyde, oxidation of ferrous ions and ultralow chemiluminescence. In protein fraction of blood serum addition of Fe2+ caused only the quick splash of chemiluminescence without the malone dialdehyde production.