Metabolism of articular cartilage in the presence of interleukin-i alpha, its inhibitor and blood serum
Isaev, P.I.; Hauselman, H.; Tonar, E.; Kuttner, K.; Zborovskiĭ, A.B.
Biulleten' Eksperimental'noi Biologii i Meditsiny 114(9): 269-271
1992
ISSN/ISBN: 0365-9615 PMID: 1477353 Document Number: 399491
The study was made of the effect which interleukin receptor antagonist protein (IRAP) and bovine serum have on interleukin-1 alpha (IL1a) activity in cartilage culture of young bulls. It was established that IL1a leads to cartilage degradation as shown by an increase of sulphated glycosaminoglycans (sGAG) in culture medium and their decrease in tissue, inhibition of proteoglycan (PG) synthesis by chondrocytes. This effect of IL1a is suppressed by IRAP. In serum-free culture the cartilage tissue produced proteoglycans in much less quantities. In these conditions IL1a stimulated chondrocytes, cytokine did not respond to IRAP.