Rat liver homogenate hydrolases splitting N-acetyl-L-tyrosine ethyl ester
Simonianova, E.; Petáková, M.; Jozová, O.; Rybák, M.
Physiologia Bohemoslovaca 24(5): 395-402
1975
ISSN/ISBN: 0369-9463 PMID: 127186 Document Number: 90348
Using gel filtration on Sephadex G 200, three fractions splitting N-acetyl-L-tyrosine ethyl ester (ATEE), termed I, S and II, were found in rat liver homogenate. In molecular size, electrophoretic mobility and thermolability, fractions I and II correspond to the ATEE-splitting enzymes contained in rat serum. The liver fraction S had a different surface electric charge and was relatively stable at high temperatures. Apart from ATEE, it splits certain ester and amide bonds of synthetic substrates. Purified fraction S increased vascular permeability in guinea pigs. Differential centrifugation of liver homogenate showed that fraction S and fractions I and II were localized differently in the subcellular particles.