Centrifugal Cytology, IV. the Prearation of fixed stained dispersions of gynecological cells
Leif, R.C.; Gall, S.; Dunlap, L.A.; Railey, C.; Zucker, R.M.; Leif, S.B.
Acta Cytologica 19(2): 159-168
1975
ISSN/ISBN: 0001-5547 PMID: 47680 Document Number: 89122
The Centrifugal Cytology technique has been utilized to produce glutaraldehyde fixed stained dispersions of both conventional Ayre scrapes and Davis pipet (PAPette) samples. Light microscope studies of dispersions of both types of cells on conventional microscope slides indicated that both the tinctorial and morphological appearance of the cells after Papanicolaou staining was very similar to that observed with conventional smears and that the same criteria could be utilized with the Centrifugal Cytology dispersions to screen the cells for cancer as had previously been used with the smears. A preliminary study indicated that six out of six positives with no false negatives or false positives were found. The Centrifugal Cytology technique appears to have promise as a method for preparing suspension samples such as pipets of gynecologic cells. Scanning electron microscope studies reveal that the squamous epithelial cells are very thin and at least some of them are covered by a network structure.