Focus assay for varicella-zoster virus in human embryo cells stained with immunoperoxidase method
Schmidtmayerová, H.; Mayer, V.; Zachar, V.
Acta Virologica 30(6): 468-474
1986
ISSN/ISBN: 0001-723X PMID: 2881466 Document Number: 284810
Rapid titration of varicella-zoster virus (VZV) in human embryonic fibroblasts (HEF) based on staining of virus-infected cells by indirect immunoperoxidase technique (IPA) is described. Cell monolayers were grown in wells of plastic plates (two different diameters). Foci of virus-infected cells as revealed by IPA could be counted either 48 hr post-infection, if cell-associated virus (VZV infected cells) was used as inoculum, or 72 hr p. i. if cell-free virus was used. A linear relationship was observed between virus dilution and number of foci. The first virus was detected 12 hr p. i., the highest titre at 36 hr, when cytopathic effect (CPE) involved about 50% of the monolayer.