Determination of the biological activity of heliomycin by a method of diffusion in agar
Kochetkova, G.V.; Matiushenkova, E.A.; Ukholina, R.S.; Kovsharova, I.N.; Uspenskaia, T.A.
Antibiotiki 20(6): 499-502
1975
ISSN/ISBN: 0003-5637 PMID: 5048 Document Number: 88851
A biological method for determination of geliomycin activity using agar diffusion is described. A nutrient medium containing Hottinger broth up to 35 mg per cent of amine nitrogen, 1.5 percent of agar-agar, tap water, pH 7.8 to 8.0 was used. Bacillus subtilis ATCC 6633 served as the test-culture. Geliomycin was dissolved in 0.1 N sodium hydroxide solution.
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