Virus replication and cell modifications in organ cultures of tumor tissue from chickens with Marek's disease
Coudert, F.; Cauchy, L.
Journal of the National Cancer Institute 55(1): 47-51
1975
ISSN/ISBN: 0027-8874 PMID: 1171990 Document Number: 86831
Organ cultures of lymphomas from chickens infected with Marek's disease (MD) virus were examined by electron microscopy at various periods after explantation. Non enveloped herpes-type virions were observed in explants 18 days after the culture was initiated. Cytolytic virus was replicated often in lymphoblastoid cells and occasionally in other cell types (permissive and nonpermissive transformed). The permissive cells were numerous, but nonpermissive transformed cells were also present as indicated by the longevity of some cultures. The presence of both permissive and transformed cell types in the organ cultures and reasons for their sequential appearance were discussed. The results unequivocally demonstrated that lymphoid cells of tumors induced by MD virus were sites of virus replication under proper culture conditions.