Top Abstract Purchase Related
Home
>
Node
>
Subnode

Effect of the method of cell disintegration on the aspartate kinase activity of preparations from Bacillus polymyxa var. Ross

Chigaleĭchuk, A.G.; Vinogradov, B.D.; Nefelova, M.V.

Prikladnaia Biokhimiia i Mikrobiologiia 11(5): 742-745

1975

ISSN/ISBN: 0555-1099
PMID: 171645
Document Number: 86668
The influence of methods of cell disintegration of Bac. polymyxa on aspartate kinase activity (EC 2.7.2.4) was examined. The disruption by means of the Hews press yielded a more active preparation as compared with ultrasonic disintegration. The supernatant treatment with streptomycin sulphate increased the preparation activity 2-fold. Dialysis of the fraction with a high activity of aspartate kinase inactivated the enzyme by 80--85%. The relationship between the aspartate kinase activity and the portein and ATP concentration in the reaction mixture was established.

Document emailed within 1 workday
Secure & encrypted payments

Pavliuk, I.V.; Bogats'kii, M.O.; Preobrazhens'kii, L.M.; Evseeva trm 1975: Polymyxin M biosynthesis by Bacillus polymyxa Ross depending on the source of ammonium nitrogen and its concentration in the medium Mikrobiolohichnyi Zhurnal 37(5): 590-593
Nefelova, M.V.; Novikova, G.A.; Egorov, N.S. 1980: Antibiotic action on Bacillus polymyxa 153 enzymatic activity Antibiotiki 25(2): 117-121
Arenas-Díaz, G.; Martínez-Rodríguez, R. 1990: Aspartate kinase as an enzyme able to phosphorylate aspartate in the nervous system. Immunocytochemical study Cellular and Molecular Biology 36(4): 395-400
Woyczikowska, B.; Paśś, L.; Girdwoyń, M.; Raczyńska-Bojanowska, K. 1973: Proteolytic degradation of polymyxins by the enzymes of Bacillus polymyxa Acta Biochimica Polonica 20(3): 285-296
Strizhkova, A.M.; Preobrazhenskiĭ, L.N.; Evseeva, R.N.; Matseliukh, B.P. 1982: Physiological characteristics of a mutant Bacillus polymyxa, a producer of polymyxin B Mikrobiolohichnyi Zhurnal 44(2): 27-29
Nefëlova, M.V.; Ermakova, G.N.; Gulevskaia, S.A.; Egorov, N.S. 1981: Electron microscopic study of Bacillus polymyxa in the developmental process and antibiotic formation Mikrobiologiia 50(4): 677-682
Emilianowicz-Czerska, W.; Niemczyk, H.; Szczepankowska, M.; Zajdel, K. 1972: Studies on biosynthesis of polymyxin e (colistin). I. Isolation and characteristics of mucoidless R-mutants of Bacillus polymyxa K-1 Acta Microbiologica Polonica. Series B: Microbiologia Applicata 4(4): 211-215
Nefelova, M.V.; Chigaleĭchik, A.G. 1971: Production of Bacillus polymyxa mutants producing the antibiotic polymyxin M using N-methyl-N-nitro-N-nitrosoguanidine Mikrobiologiia 40(6): 1084-1087
Kit, I.I.; Iakubov, L.A.; Kuligina, E.V.; Rikhter, V.A. 2003: Interaction of oligonucleotides and ATP with preparations of sIgA possessing protein kinase activity Ukrains'kyi Biokhimichnyi Zhurnal 75(2): 62-66
Shapiro, A.; Ratliff, T.L.; Oakley, D.M.; Catalona, W.J. 1983: Reduction of bladder tumor growth in mice treated with intravesical Bacillus Calmette-Guérin and its correlation with Bacillus Calmette-Guérin viability and natural killer cell activity Cancer Research 43(4): 1611-1615
Alekseev, A.N.; Sokolova, E.I.; Kosovskikh, V.L.; Khorkhordin, E.G.; Rasnitsyn, S.P. 1983: Effect of the larval density of Aedes aegypti mosquitoes on their mortality as affected by Bacillus thuringiensis Berl. preparations Meditsinskaia Parazitologiia i Parazitarnye Bolezni 52(1): 78-80
Chapekar, M.S.; Hartman, K.D.; Knode, M.C.; Glazer, R.I. 1987: Synergistic effect of retinoic acid and calcium ionophore A23187 on differentiation, c-myc expression, and membrane tyrosine kinase activity in human promyelocytic leukemia cell line HL-60 Molecular Pharmacology 31(2): 140-145
Méndez-Samperio, P.; Pérez, A.; Trejo, A. 2007: Sphingosine kinase, phosphatidylinositol 3-kinase, Akt, NF-kappaB, and p300 are required for CCL5 production in Mycobacterium bovis Bacillus Calmette-Guérin (BCG)-infected epithelial cells Cellular Immunology 249(2): 94-100
Gumińska, M.; Wazewska-Czyzewska, M. 1980: Effect of Magnesium Diasporal on erythrocyte pyruvate kinase activity in healthy persons and in patients with pyruvate kinase deficiency Folia Medica Cracoviensia 22(3-4): 425-433
Tarnowski, R. 1973: Effect of 17-estradiol on the activity of aspartate carbamoyl-transferase (EC 2.1.3.2.) in the liver, kidney and uterus of the rat Ginekologia Polska 44(9): 985-991
Polikarpova, L.I. 1973: Effect of insulin on the activity of aspartate- and alanine-transaminases in the isolated liver of normal rats and rats with alloxan diabetes. Effect of castration Problemy Endokrinologii 19(1): 55-57
Jungowska-Klin, B.; Lozińska, M.; Wojtaszek, J. 1975: The effect of X-irradiation on the alanine--and aspartate aminotransferase activity in the liver, kidney and spleen of mouse Acta Physiologica Polonica 26(1): 95-101
Puchkov, E.O.; Govorunova, V.A.; Babaeva, P.V. 1983: Effect of gramicidin D on the exoprotease activity of Bacillus thuringiensis Prikladnaia Biokhimiia i Mikrobiologiia 19(5): 599-602
Bowers, G.N.; McComb, R.B.; Syed, D.; Edwards, G.C.; Edwards, J.; Paule, R.C.; Greenberg, N.; Mauck, L.A.; Ferris, R.J.; Hørder, M. 1988: Primary human aspartate aminotransferase reference material. The catalytic activity concentration of AST in RM 8430 as measured by the IFCC reference method for AST in 10 enzyme standardization laboratories Clinical Chemistry 34(2): 450-459
El-Hamalawi, A.R.; Shihabi, H.R. 1984: Effect of polycations on the transforming activity of various markers of Bacillus subtilis Italian Journal of Biochemistry 33(6): 369-380