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Effect of polycations on the transforming activity of various markers of Bacillus subtilis

El-Hamalawi, A.R.; Shihabi, H.R.

Italian Journal of Biochemistry 33(6): 369-380

1984

ISSN/ISBN: 0021-2938
PMID: 6441790
Document Number: 237332
Polycations interact with DNA and form DNA-polycation complexes. The soluble and insoluble fractions of the DNA-polycation complexes were fractionated by centrifugation. The effect of polycations on the transforming activity of genetic markers of B. subtilis (single and double) have been tested. These markers are tryptophan, histidine, adenine, arginine and lysine. It is found that both parts of DNA in the supernatant and the precipitate of DNA-polycation complexes are biologically active. This means that the activity of the precipitated genetic markers by the different polycations was reserved.

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