Influence of the oxygenation state of the culture media on the level of non esterified fatty acids and of cholesterol in heart cells of the newborn rat
Freyss-Beguin, M.; Mathieu-Levy, N.; Brussel, E.V.; Lechat, P.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 169(6): 1429-1435
1975
ISSN/ISBN: 0037-9026 PMID: 132232 Document Number: 85556
The uptake of non esterified fatty acids (NEFA), from a medium supplemented with 10% of calf serum, by rat myocardial cells cultured during 8 days, without renewal of the medium, was identical whatever the values of partial pressures of oxygen in the medium. Cellular cholesterol level was reduced when air or oxygen (+5% CO2) was blown into culture bottles; it was enhanced by gassing with nitrogen 24 h later. Cellular NEFA level was reduced by air, but not by oxygen blowing; it was enhanced by gassing with nitrogen 24 h later. The variations observed may originate from pertubations of NEFA beta-oxidation.