A method for culture and recovery of gastrointestinal strongyle larvae

Henriksen, S.A.; Korsholm, H.

Nordisk Veterinaermedicin 35(11): 429-430

1983


ISSN/ISBN: 0029-1579
PMID: 6672763
Document Number: 85
In 1975 Sellers el al. described a technique for culture and recovery of the third stage larvae of llyostrangylas rubidas and Oesophagostomum spp. from porcine faeces samples: Faeces were mixed with vermiculite and placed in a vessel made from a plastic drinking cup that had been shortened so as to hold between 60 and 65 ml. The opening of the vessel was covered with surgical lint, fastened by a rubber band. The vessel was placed in a reversed position inside an intact disposable cup to which 5-10 nil of water had been added. After incubation for seven days at 28°C the larvae were recovered by filling up the intact cup with water, allowing the larvae to migrate through the lint and sink to the bottom of the cup.

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A method for culture and recovery of gastrointestinal strongyle larvae