Cellular capacity in tissue culture and in tumors for recovery from the injuries induced by N-methyl-N-nitrosourea
Chan Tik Canh; Gotlib, V.Ia.; Afanas'ev, G.G.; Pelevina, I.I.
Tsitologiia 21(10): 1217-1221
1979
ISSN/ISBN: 0041-3771 PMID: 505572 Document Number: 146141
The survival of the LL cell line in the log-phase of growth, and clonogeneic ability of NKLy/LL tumours, derived from a subcutaneous transplantation of these cells to mice, have been investigated. The survival assay of culture cells and cells in tumours was studied both immediately, and 6--72 hours after the treatment with N-methyl-N-nitrosourea (MNU) in concentrations of 0.05 mg/ml in vitro, and of 12.5--200.0 mg/kg injected in vivo. No repair of potentially lethal damages was noticed in tissue culture during a 72 hours incubation in conditioned medium after the MNU treatment, or in small NKLy/LL tumours when analysed 24 hours after the drug injection. Possible mechanisms of an irrepairable damage induction are discussed.