Comparative histochemical and disc-electrophoretical investigations about the postnatal differentiation of the alkaline and acid phosphatases in the intestinal mucosa of the rat
Höhn, P.; Schäfer, A.; Gabbert, H.; Paasch, W.
Acta Histochemica 54(2): 200-217
1975
ISSN/ISBN: 0065-1281 PMID: 814768 Document Number: 83434
The alkaline and acid phosphatase were studied with different histochemical methods in 5 different regions of the small intestine in newborn (1. to 50. postnatal day) and in adult rats (4 month old) and compared with the results of the disk-electrophoretical fractionation of these enzymes. In the course of the adaptation of the enzymes pattern of newborn animals to the pattern of adult animals during the 3. postnatal week, in the disk-elektrophoresis there is a alteration of the qualitative composition of the enzymes. Until the 20. day the electrophoresis shows only one fraction of alkaline and acid phosphatase in duodenum. After this day three fractions can be separated. Simultaneously there is a precipitous decrease in the enzyme activities in the electropherograms of the ileum. Synchronous with the enzyme adaptation the characteristic surface epithelium of ileum is replaced by non vacuolised cylindrical epithelium.