Separation of glycopeptides derived from brain glycoproteins by gel filtration
Benedetta, C.D.; Brunngraber, E.G.
Italian Journal of Biochemistry 24(2): 97-101
1975
ISSN/ISBN: 0021-2938 PMID: 1158653 Document Number: 83235
Glycopeptides obtained from rat brain by proteolytic digestion with papain have been separated from glycosaminoglycans by means of gel filtration. The glycosaminoglycans appear in the void volume, whereas the glycopeptides are retarded. Glycopeptides of groups A+B (Brunngraber et al., 1973) (MW = 3800-500) C+D (MW = 2000) which were partially resolved by the method, were identified in the elution profile. Nucleic acids, also solubilized by papain, are eluted together with the glycosaminoglycans.
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