Electrophoretic separation and properties of acid phosphatase isoenzymes from rat brain
Stach, E.; Ockenfels, H.; Pilgrim, C.
Acta Histochemica 60(1): 121-131
1977
ISSN/ISBN: 0065-1281 PMID: 413317 Document Number: 124211
Following agar gel electrophoresis of the 20,000 .times. g supernatant of rat brain homogenate a single band with acid phosphatase activity could be visualized by means of azo dye techniques. By adding Triton X-100 to the homogenate, a 2nd band with enzymatic activity was demonstrable. A 3rd isoenzyme could be identified in the presence of Zn2+. A 4th band visible only when employing Pb methods was considered an artifact as its appearance did not depend on the presence of a substrate. The substrate specificity of the isoenzymes and the possibilities of influencing their activity were examined. By specific choice of substrates and effectors, conditions could be defined rendering possible a selective identification of the individual isoenzymes.