In vivo incorporation of labelled precursors into lipids of liver & kidneys of mice during postnatal development
Pushpendran, C.K.; Eapen, J.
Indian Journal of Experimental Biology 12(6): 484-486
1974
ISSN/ISBN: 0019-5189 PMID: 4464249 Document Number: 80264
Swiss mice 1 and 75 days old were studied. Mice of 1, 5 and 10 days were suckled and used in the experiment within 15 min of being removed from the mother. From 20 days old mice received a standard diet and were given intraperitoneally palmitate-1-14C, acetate-1-14C or glucose-U-14C and killed at intervals from 15 to 120 min later. The liver and kidneys were removed. For 1-, 5- and 10-day-old mice tissues were pooled. Tissue samples were homogenised and the lipids were extracted. Total lipid and radioactivity were estimated, then the lipid was fractionated by thin-layer chromotography and the radioactivity in each fraction was estimated. Incorporation of palmitate and glucose into mice was dependent on age; the older the mice the greater the incorporation into both liver and kidney. The incorporation of glucose was highest 30 min after injection in mice 5 days old. In 30-day-old mice incorporation of palmitate, glucose and acetate was highest in both liver and kidney 15 min after injection. In 75-day-old mice the incorporation into kidney was similar to that in 5-day-old mice but the incorporation into liver was similar to that in 30-day-old mice. Radioactivity in both hepatic and renal lipids was associated mainly with the phospholipid fraction except for a higher proportion in the triglyceride fraction of the hepatic lipid after palmitate injection.