Immunization of cattle with apolyvalent bovine adenovirus vaccine
Bartha, A.
Developments in Biological Standardization 26: 15-18
1974
ISSN/ISBN: 0301-5149 PMID: 4373297 Document Number: 71255
From serological investigations it was supposed that any virus type belonging to the 1st (kidney) subgroup or the 2nd (testicle) subgroup of bovine adenoviruses can confer immunity against all other types of the same subgroup, but is not able to give protection against types of the other subgroup. A formalin inactivated vaccine was prepared from type 1 (1st subgroup) and type 8 (2nd subgroup) bovine adenoviruses. These virus types were propagated in bovine testicle cell cultures and were considered suitable for vaccine production if their titres were higher than 103 TCID/50 per 0.1 ml. Serologic conversion range of sera of animals inoculated with 2 ml vaccine was from 1:2 to 1:32. Cows inoculated twice in late pregnancy immunized their calves passively through the colostrum. Calves with colostral antibodies were vaccinated 2 months after birth, and calves free from antibodies were vaccinated as early as possible (10 days after birth).