Tissue culture and animal studies with an oncolytic bovine enterovirus (bovine enterovirus 1)
Hodes, M.E.; Morgan, S.; Hubbard, J.D.; Yu, P.L.; Lukemeyer, J.W.
Cancer Research 33(10): 2408-2414
1973
ISSN/ISBN: 0008-5472 PMID: 4355026 Document Number: 69941
Treatment of mouse cells derived from Sarcoma 180, Ehrlich ascites, A755, methylcholanthrene-induced epithelial carcinoma, and an L-cell line in micro-test plates with bovine enterovirus type 1 caused a decrease (18 to 98%) in viable cells, whereas cells derived from spleen, lung, thymus, liver, and kidney of normal mice did not show any change after exposure to virus. The susceptibility of rabbit and mouse kidney cells to BEV did not change after 3 (mouse) to 12 (rabbit) passages in culture. However, the 'normal' cells of the continuous lines Vero, BSC-1, and LLC-RK were susceptible to the virus in both the microtest plate and plaque assays. Normal mice, rabbits, and dogs with BEV-1 by intracranial injection failed to show any clinical abnormalities, nor could virus be recovered from the brain. Some inconstant changes were noted on pathological examination. Mice bearing Sarcoma 180 and treated by i/p injection of BEV-1 showed only mild splenic hyperplasia early in the course of treatment.