Release of immunosuppressive substances from tissue culture cells infected with bovine viral diarrhea virus
Markham, R.J.; Ramnaraine, M.L.
American Journal of Veterinary Research 46(4): 879-883
1985
ISSN/ISBN: 0002-9645 PMID: 2990265 Document Number: 250950
Bovine fetal lung tissue culture cells were infected with bovine diarrhoea virus. The cells released substances into the supernatant fluid that suppressed the proliferative response of bovine peripheral blood mononuclear cells which had been stimulated with concanavalin A. Activity was present in low molecular weight fractions. During the assay, these fractions were required to inhibit the response of leukocytes to concanavalin A. Addition of indomethacin to the infected tissue culture cells inhibited generation of immunosuppressive supernatants; addition of indomethacin to the suppressive supernatants did not block their activity. Involvement of prostaglandins in this immunosuppressive activity is discussed.