Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. a comparative examination using CF, PLA and NPLA assays
Jensen, M.H.
Acta Veterinaria Scandinavica 22(1): 85-98
1981
ISSN/ISBN: 0044-605X PMID: 6266240 Document Number: 174169
The antibodies in serum samples from an outbreak of low-virulent hog cholera in Spielbach, West Germany, in 1966, and serum samples from pigs inoculated with hog cholera (HC) virus and bovine viral diarrhea (BVD) virus, respectively, were examined by 3 different methods: a modified direct complement fixation (CF) test; a peroxidase-linked antibody (PLA) assay based on microplates with fixed, viral-antigen containing cells; and a neutralization assay carried out in microplates using the chessboard principle and read by means of the peroxidase-linked antibody (NPLA) assay. A good correlation was found in their ability to detect the antibodies. Generally neutralizing antibodies were found 2 wk after inoculation. By CF and PLA, antibodies were detected at the same time or up to 2 wk later. All sera were tested by the 3 methods against the HC viral antigen and BVD viral antigen. HC-antibodies were not distinguished from BVD-antibodies by CF; they were distinguished to a certain degree by PLA. BVD-antibodies were to a certain degree distinguished from HC-antibodies by CF but not by PLA. Thus, CF and PLA together can differentiate between the 2 types of antibodies. NPLA differentiated HC-antibodies and BVD-antibodies.