Indirect immunofluorescence using F (ab') 2-immunoreagents for the demonstration of bovine viral diarrhea virus (BVDV) antigen in lymphoid tissue
Ohmann, H.B.; Dalsgaard, K.
Acta Veterinaria Scandinavica 21(4): 705-707
1980
ISSN/ISBN: 0044-605X PMID: 7013454 Document Number: 160435
In studies on bovine diarrhoea virus antigen localization in lymphoid tissue and replication of the virus in bovine macrophages, using antibody from which the Fc portion had been removed by pepsin, indirect immunofluorescence of infected bovine macrophage cultures, unfixed or fixed in 20% acetone, showed that the antigen was confined to the cell cytoplasm.
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