In vitro studies of bone resorption by the root-resorbing tissue from the bovine deciduous tooth
Oguchi, H.
Bulletin of Tokyo Medical and Dental University 22(2): 175-183
1975
ISSN/ISBN: 0040-8921 PMID: 1064488 Document Number: 93766
Granulation tissue which is responsible for root resorption of deciduous tooth lies between root of the deciduous tooth and its permanent tooth germ. This tissue is called "root-resorbing tissue". Its bone-resorbing activity was investigated in vitro. Bovine root-resorbing tissue was cultured in close contact with 45Ca-labeled dead calvaria of rats. Bone-resorbing activity was determined by measuring 45Ca released from labeled calvaria during culture. It was found that only the root-resorbing tissue which was rich in odontoclasts and had a good blood supply in its surface layer had bone-resorbing ability, and that bone resorption occurred only when it was placed in close contact with calvarium. The root-resorbing tissue which was poor in odontoclasts and blood vessels failed to stimulate by 25-hydroxy-vitaim D3 or heparin, but not by larger amounts of parathyroid hormone, vitamin D3, and dihydrotachysterol when added to the culture.