Hplc Method for Separating Enantiomers of Imidazole Derivatives - Antifungal Compounds
Podolska, M.; Bialecka, W.; Kulik, A.; Kwiatkowska-Puchniarz, B.; Mazurek, A.
Acta Poloniae Pharmaceutica 74(3): 777-784
2017
ISSN/ISBN: 0001-6837 PMID: 29513946 Document Number: 693654
The aim of this study was to test separation possibility of enantiomers of nine active substances belonging to imidazole derivatives: bifonazole, butoconazole, econazole, enilconazole, fenticonazole, isoconazole, miconazole, sertaconazole and tioconazole. The study was performed using HPLC method and the CHI- RALCEL OJ column (10 gm; 250 x 4.6 mm), the mobile phase flow rate of 0.8 mL/min and detection at 220 rim. Mobile phases containing hexane and the following modifiers: alcohols (2-propanol, ethanol, methanol) and diethylamine were tested. At first isocratic elution was used but some enantiomers eluted after a long retention time and their peaks were asymmetrical and too wide. Therefore, a gradient elution was developed allow- ing to obtain satisfactory retention times and other parameters of enentioseparation of the compounds.