Application of the MALDI-TOF for identification of Clostridium perfringens strains
Brodzik, C.; Augustynowicz, E.; Korzeniowska-Kowal, A.; Lutyhska, A.
Medycyna Doswiadczalna i Mikrobiologia 68(1): 13-21
2016
ISSN/ISBN: 0025-8601 PMID: 28146618 Document Number: 690561
The anaerobic bacilli Closfridium perfringens are commonly found in soil and sewage and are also part of the gastrointestinal tract of humans and animals. Food poisoning caused by C. perfringens are regarded the most common diseases transmitted by contaminated food in the world. Bacteria of this species due to their pathogenic properties sustain the constant object of studies in order to elucidate mechanisms of toxinogenesis, to determine the roads of transmission and to develop better diagnostic tools. The objective of the study was to verify, the suitability of the MALDI-TOF method for the identification of C. perfringens species. In order to identify anaerobic bacteria C. perfringens MALDI-TOF method, sequencing of 16S rRNA and detection of cpe and cpa gene by duplex PCR were used. MALDI-TOF confirmed C. perfringens identification in 39 isolates out of 41 archival isolates under study. The correctness of the results was verified by 16S rRNA sequencing and cpa gene detection. The study confirmed the usefulness of MALDI-TOF method in rapid identi- fication of anaerobic bacteria C. perfringens and thereby its applicability epidemiological investigations.