The time-of-flight mass spectrometer intra-specific diagnostic of strains of Escherichia isolated from patient
Gapon, M.N.; Telesmanitch, N.R.; Ternovskaya, L.N.; Chaika, S.O.; Chaika, I.A.; Mikashinovitch, Z.I.; Tverdokhlebova, T.I.
Klinicheskaia Laboratornaia Diagnostika 61(6): 371-375
2016
ISSN/ISBN: 0869-2084 PMID: 30601631 Document Number: 690340
The time-of-flight mass spectrometer analysis of freshly separated human colibacillus populations for identification of markers of proteom specific for hemolytic and non-hemolytic strains, Escherichia-associant of opportunistic enterobacteria. The material consisted of 20 strains of mono-population of hemolytic Escherichia; 100 strains of mono-population of non-hemolytic Escherichia; 15 non-hemolytic strains isolated from association with opportunistic enterobacteria. The protein profiling was implemented on mass spectrometer MALDI-TOF MS Autoflex "Bruker Daltonik". The value within type of peak mass-charge with 100% intensity equal to 9000 Da can be used as a differentiating indication of detection of hemolytic Escherichia at mass-spectrometer biotyping. The proteom characteristics of strains of various populations within Escherichia coli species are given. The taxon-specific markers for proteom differentiation of population of Escherichia are given too.