Proteomic Mass-Spectrometric Analysis of Freshly Isolated Escherichia Coli Strains
Gapon, M.N.; Telesmanich, N.R.; Ternovskaya, L.N.; Chaika, S.O.; Chaika, I.A.; Mikashinovich, Z.I.; Chernukhina, T.B.; Tverdokhlebova, T.I.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2015(3): 83-88
2015
ISSN/ISBN: 0372-9311 PMID: 26259276 Document Number: 681737
Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli--associants of opportunistic bacteria. 20 strains of monopopulation of hemolytic E.coli; 100 strains of monopopulation of non-hemolytic E. coli; 15 non-hemolytic strain, isolated from association with opportunistic bacteria. Protein profiling on MALDI-TOF MS Autoflex "Bruker Daltonik" mass-spectrometer. Value within species for mass-charge peak with 100% intensity of 9000 Da could be a differential sign for isolation of hemolytic E.coli during mass-spectrometric biotyping. Proteomic characteristics of strains from various populations within E.coli are given. Taxon-specific markers for proteomic differentiation of E.coli populations are established.