Differentiation of Escherichia coli strains isolated from patients with urinary tract infection based on biochemical properties, antibiogram and colicinogenic typing
Galiński, J.; Misiewicz, I.; Nowicki, B.
Medycyna Doswiadczalna i Mikrobiologia 36(2): 107-112
1984
ISSN/ISBN: 0025-8601 PMID: 6387330 Document Number: 229748
Biochemical colicinogenic typing and antibiographs were evaluated as methods for the differentiation and identification of E. coli strains in chronic urinary tract infections. A group of 127 strains were tested, isolated from the urine of 35 patients in a 3.5-mo. observation period. The number of cultures isolated from each patient ranged from 2 to 7. The biochemical series included rhamnose, sucrose, raffinose and dulcitol fermentation, lysine and ornithine decarboxylation, determination of mobility and hemolysis. Colicinogenic typing was performed with a kit of 10 indicator strains (Abbott-Shannon). Drug resistance was determined with 9 drugs used in therapy. In 60% of the patients bacterial cultures obtained from the consecutive urine samples were identical in each of the tests applied. E. coli isolated from the other 40% of patients showed differences detectable by at least one of the tests. Biochemical typing appeared the best method for the differentiation of E. coli strains in chronic urinary tract infections. Drug resistance determination differentiated a lower number of strains, but the results could always be confirmed by other tests. High percent of strains that could not be typed was a disadvantage of colicinogenic typing.