Novel Approach to Composition Of, Bacteriophage Mixtures for Antibacterial Therapy
Pleteneva, E.A.; Shaburova, O.V.; Burkaltseva, M.V.; Krylov, S.V.; Kaplan, A.M.; Chesnokova, E.N.; Polygach, O.A.; Voroshilova, N.N.; Mikhailova, N.A.; Zverev, V.V.; Krylov, V.N.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 3-11
2016
ISSN/ISBN: 0372-9311 PMID: 30695342 Document Number: 689482
Evaluate antibacterial activity of an experimental mixture of phages, belonging to several well-studied species. The study was carried out using a group of 55 clinical Pseudomonas aeruginosa strains of various origins,- 4 mono-species mixtures of 32 virulent bacteriophages (species phiKZ-, phiKMV-, phiPBl-, PaP3-like phages) and 2 novel phages, phiMK (species PaK-P2) and phiPerm5. Activity of preparations from mono-species mixtures of bacteriophages ofvarious species were compared with activity of 3 commercial mixtures. Standard methods of study of bacteriophages were used: determination of lytic activity by seeding onto bacterial lawns of P. aeruginosa, restriction analysis of phage DNA for confirmation of their be- longing to certain species. Cumulative activity of 6 mono-species mixtures of virulent phages was shown to be similar to lytic activity of commercial therapeutic mixtures used against P. aeruginosa infections. 54 of 55 strains of clinical isolates of P: aeruginosa showed sensitivity to experimental mixtures composed of mono-species mixtures of bacteriophages. 53 strains were lysed by commercial preparations. Wherein the possibility of accidental inclusion of moderate -bacteriophages in the experimental mixture is excluded. A possibility of creation of highly active therapeutic antibacterial preparations against P. aeruginosa using mono-species mixtures of 6 species of lytic bacteriophages is shown Use of such a mixture in therapy of lung infections reduces the risk of emergence of bacterial strains with increased virulence and patho- genicity during prolonged administration.