Development and evaluation of the RT-PCR kit for the rabies virus diagnosis
Dedkov, V.G.; Deviatkin, A.A.; Poleschuk, E.M.; Safonova, M.V.; Markelov, M.L.; Shipulin, G.A.
Voprosy Virusologii 61(5): 235-240
2016
ISSN/ISBN: 0507-4088 PMID: 29323857 Document Number: 687022
To improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus DNA by the RT-PCR technique was developed and evaluated. The analytical sensitivity of the kit was 4*10 GE per ml. High specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.
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