Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci
Dong, Y.; Lin, S.-s.; Cao, Y.; Wu, W.-w.; Huang, S.-q.; Zheng, W.-g.; Li, F.-y.; Ge, B.-w.; Guo, Y.-l.; Zhou, H.-g.
Fa Yi Xue Za Zhi 31(5): 373-376; 380
2015
ISSN/ISBN: 1004-5619 PMID: 26821481 Document Number: 680315
To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice. Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed. A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin. The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.