Detecting haplotypes of four Y-STR by multiplex amplification
Liu, Q.-l.; Lu, D.-j.; Xu, N.-M.
Fa Yi Xue Za Zhi 21(1): 9
2005
ISSN/ISBN: 1004-5619 PMID: 15895799 Document Number: 593233
To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population. DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining. When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853. The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.