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Development and application of X-chromosomal STR multiplex amplification system

Liu, Q.-L.; Lü, D.-J.; Zhao, H.

Fa Yi Xue Za Zhi 25(6): 425-427

2009

ISSN/ISBN: 1004-5619
PMID: 20225617
Document Number: 633599
To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing. Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software. The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility. The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.

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