The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase

Shershov, V.E.; Kuznetsova, V.E.; Lysov, Y.P.; Guseinov, T.O.; Barsky, V.E.; Spitsyn, M.A.; Zasedateleva, O.A.; Vasiliskov, V.A.; Surzhikov, S.A.; Zasedatelev, A.S.; Chudinov, A.V.; Shershov, V.E; Kuznetsova, V.E; Lysov, Y.P; Guseinov, T.O; Barsky, V.E; Spitsyn, M.A; Zasedateleva, O.A; Vasiliskov, V.A; Surzhikov, S.A; Zasedatelev, A.S; Chudinov, A.V

Home

>

Node

>

Subnode

The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase

Shershov, V.E.; Kuznetsova, V.E.; Lysov, Y.P.; Guseinov, T.O.; Barsky, V.E.; Spitsyn, M.A.; Zasedateleva, O.A.; Vasiliskov, V.A.; Surzhikov, S.A.; Zasedatelev, A.S.; Chudinov, A.V.

Biofizika 60(6): 1216-1218

2015

ISSN/ISBN: 0006-3029

PMID: 26841520

Document Number: 679601

In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA during PCR, three fluorescently-labeled dUPT, one of which with electroneutral and other two with positively and negatively charged dyes (Cy5 analogs), were synthesized. It is shown that dUPT, labeled with electroneutral Cy 5 analog, is most effectively incorporated into DNA when Tag polymerase is used for PCR.