Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides
Sällström, J.F.; Zehbe, I.; Alemi, M.; Wilander, E.
Anticancer Research 13(4): 1153
1993
ISSN/ISBN: 0250-7005 PMID: 8394673 Document Number: 420021
False positivity is reported of in situ PCR reactions in a direct incorporation assay with digoxigenin-labelled dUTP. It is recommended that in situ hybridization with specific labelled probe replaces the direct incorporation method for the detection of in situ PCR amplicon.
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