Proliferation inhibition and apoptosis induction of Juglone on human cervical cancer Caski cells
Zhang, W.; Li, Y.; Luo, J.; Zhao, X.; Xu, J.; Zhu, W.; Jiang, Y.; Fang, F.
Wei Sheng Yan Jiu 43(6): 959
2014
ISSN/ISBN: 1000-8020 PMID: 25603606 Document Number: 678282
To explore the effects of Juglone on proliferation and apoptosis of human cervical cancer Caski cells, and to further study the related mechanism of cell apoptosis. Cultured Caski cells were incubated with 20, 40, 60, 80 and 100 μmol/L juglone for 24 h. The proliferation of Caski cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The cell apoptosis were detected by transmission electron microscope. The expression of Bcl-2 and Bax were detected by Western blot. MTT results showed that in different doses of juglone groups, the Caski cell growth was greatly inhibited (P < 0.05, P < 0.01) and showed dose dependent when compared with control group except 20 μmol/L. The IC50 of juglone was 42.4 μmol/L. After treatment on Caski cells with 40 μmol/L juglone, typical apoptosis characteristics was observed by transmission electronmicro scope. The expression of Bcl-2 was decreased while the expression of Bax was increased significantly when compared with control group (P < 0.05). Juglone significantly inhibits the proliferation and induces the apoptosis of Caski cells in vitro.