Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells
Peng, P.; Huang, L.; Wang, Y.; You, C.; Cao, W.; Song, H.; Tan, H.; Wu, Y.
Zhongguo Zhong Yao Za Zhi 36(18): 2539-2542
2011
ISSN/ISBN: 1001-5302 PMID: 22256762 Document Number: 654988
To observe the effects of high expression of recombinant trichosanthin (rTCS) on the cell proliferation and cell cycle of human cervical cancer Caski cells. Eukaryotic expression plasmid pcDNA3.1(-)/6His-TCS was constracted and stably transfected into Caski cells. RT-PCR,Western-blot were used to select the clones with rTCS high-expressing. Using pcDNA3.1(-)-transfected cells as the control, MTT assay and flowcytometry were used to elucidate the effects of rTCS high expression on cell growth and cycle regulation in Caski cells. The Caski cells with stable high expression of rTCS was successfully established, which could inhibit the cell growth (P<0.01) and arrest Caski cells in G1 and G2 phases (P<0.05) obviously. High expression of rTCS can inhibit the growth of Caski cervical cancer cells, which might provide a new pathway for the therapy of cervical cancer.