IL-10 and trichosanthin inhibited surface molecule expression of antigen processing cells and T-cell proliferation
Fan, Z.S.; Ma, B.L.
Zhongguo Yao Li Xue Bao 20(4): 353-357
1999
ISSN/ISBN: 0253-9756 PMID: 10452124 Document Number: 498717
The immunoinhibitory effects and preliminary mechanism of IL -10 and trichosanthin (isolated from Trichosanthes kirilowii) were investigated. Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analysed by FACScan. B7-1 mRNA expression was detected with nested RT-PCR. IL-10 (2 mg/litre) and trichosanthin (10 mg/litre) inhibited B7-1 molecule expression. IL-10 and trichosanthin down-regulated LFA-1 expression, but had no regulatory effect on CD40. IL-10 and trichosanthin dramatically inhibited T-cell proliferation and IL-2 production. B7-1 mRNA expression was undetectable in APC treated with IL-10 and trichosanthin. IL-10 and trichosanthin inhibited surface molecule expression on APC.