Effects of high-fat diet on FABP2 mRNA expression in small intestinal tissue of young rats
Xu, Y.; Wang, W.; Hao, M.; Zhang, X.; Gao, X.; Chang, X.
Wei Sheng Yan Jiu 43(5): 749-753
2014
ISSN/ISBN: 1000-8020 PMID: 25438528 Document Number: 676197
To measured FABP2 mRNA expression of small intestinal rats in response to high-fat diet; and to explore the correlation between expression and child obesity in rats. The young obese rat models high-fat diet-induced method. Then the expression levels of FABP2 mRNA jejunum and ileum tissues of young rats were detected and analyzed using real time fluorescence quantitative PCR method. The serum biochemical assessed with an automatic biochemical Analyzer. In both rnd obesity group, the expression levels of FABP2 mRNA were highest in ermediate in the jejunum, and lowest in the duodenum both the control obesity group, there' were significant differences among them ( P <0. 05). Compated with control group, the expression levels of FABP2 mRNA in obesity group were significantly decreased (P <0.05); and the levels of blood glucose and serum TG in the obesity group were significantly higher (P < 0.05 ). FABP2 mRNA expression was different in diverse segments of intestinal tissue. Feeding high-fat diets allowed rats to reduce the expression levels of FABP2 mRNA in small intestinal tissue, meanwhile, the rats gained weight and elevate serum TG levels. It suggests that FABP2 involves in lipid metabolism, and is associated with the development of childhood obesity.