Effects of high-fat diet on blood lipids in rats
Guo, F.; Li, Y.; Sun, C.; Niu, Y.; Feng, R.
Wei Sheng Yan Jiu 40(1): 40-42
2011
ISSN/ISBN: 1000-8020 PMID: 21434309 Document Number: 651279
To provide a basis for establishing better animal models with hyperlipidemia by comparing the effects of two different high-fat diets on blood lipids of rats. 30 male Wistar rats were randomly divided into 3 groups (10 rats in each group) , including normal control group (NC), high fat formula diet group I (HFFD I) and high fat formula diet group II (HFFD II). NC group was fed a stock rodent diet, the HFFD I and HFFD II were fed with different high-fat formulae diets. Animals were weighted weekly and blood lipids were determined every two weeks. The experiment lasted for 6 weeks. By the end of the experiment, serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in HFFD I group were 1.4 and 2.2 times higher than those of the NC group (P < 0.01). Serum high-density lipoprotein cholesterol (HDL-C) was 22.2% lower than that of NC group. Serum triglyceride (TG) was not significantly different between HFFD I and NC group (P > 0.05). Serum TC and LDL-C of HFFD II group were 11.5 and 5.7 times respectively higher than those of the NC group (P < 0.01). Serum TG and HDL-C were 39.7% and 34.6% respectively lower than those of the NC group. Animal models with hypercholesteremia can be successfully established by the two methods, but the serum TG level of the rats can not be significantly elevated.