Investigation into the vestibular protective properties of melatoninergic agents
Iasnetsov, V.V.; Motin, V.G.; Karsanova, S.K.; Iasnetsov, V.V.; Ivanov, I.V.; Chel'naia, N.A.
Aviakosmicheskaia i Ekologicheskaia Meditsina 48(2): 31-34
2014
ISSN/ISBN: 0233-528X PMID: 25087409 Document Number: 674400
Experiments with rats showed that melatonin (2.5 mg/kg) produces a distinct vestibular protective effect excelling promethazine (50 mg/kg) as a reference agent, and also antidepressant agomelatine (5 mg/kg) as another melatoninergic agent. Lusindol, a blocker of MT1/MT2-receptors (2.5 mg/kg), and bicuculline (1.5 mg/kg), a specific GABA-receptors antagonist, weakened the melatonin effect significantly. The results testify mediation of the melatonin action by these receptors. Whole-cell patch clamp in an experiment with convoluted oblongata sections from white nonlinear infant male rats (14-d old) disclosed that melatonin (2 mM) inhibited drastically (29 +/- 3%) the excitatory postsynaptic current caused by depolarization step in neurons of the medial vestibular nucleus. Lusindol (0.1 mM) inhibited the effect of melatonin (2 mM) significantly (71 +/- 6%) which suggests involvement of melatonin MT1/MT2-receptors.