MALDI-TOF mass-spectrometric analysis in the accelerated identification of the Vibrio genus microorganisms
Afanasev, M.V.; Mironova, L.V.; Basov, E.A.; Ostyak, A.S.; Kulikalova, E.S.; Urbanovich, L.Y.; Balahonov, S.V.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 3: 22-29
2014
ISSN/ISBN: 0208-0613 PMID: 25335409 Document Number: 672980
The goal of this work was to develop methodological approaches to identification of the Vibrio genus representatives using the MALDI-TOF mass-spectrometric analysis technologies. The aspects of the biological safety in sample preparations for mass-spectrometric analysis were studied, reference spectra of six typical V. cholerae strains were developed. Identification of 55 strains, representatives of the Vibrio genus, including 45 V. cholerae strains with different epidemic importance, was performed using the MALDI Biotyper 3.0 basis comprising V. cholerae reference spectra. The possibility of reliable definition of the tested strain taxonomic belonging to the species level was demonstrated. Thus, the results completely corresponded to the data of classical microbiological identification. Stability and reproducibility of the offered research method was experimentally shown. The results allow identification of the Vibrio genus representatives to be implemented with the use of the mass-spectrometric analysis as an effective method that defines a species belonging of the basic Vibrio genus representatives in the shortest-terms.