Identification of transthyretin posttranslational modifications 1n human blood using mass-spectrometric methods
Popov, I.A.; Starodubtseva, N.L.; Indeĭkina, M.I.; Kostiukevich, I.I.; Kononikhin, A.S.; Nikolaeva, M.I.; Kukaev, E.N.; Kozin, S.A.; Makarov, A.A.; Nikolaev, E.N.
Molekuliarnaia Biologiia 47(6): 1011-1019
2013
ISSN/ISBN: 0026-8984 PMID: 25509863 Document Number: 665734
Transthyretin, one of the major plasma proteins, has a number of posttranslational modifications and mutations, some of which are associated with the development of severe diseases, for instance, familial amyloid neuropathy and Alzheimer's disease. In order to investigate the role of modified forms in the development of these diseases a complex analytical platform, based on two mass-spectrometric approaches (bottom-up and op-down) has been developed. The high efficiency of this method was shown using 10 plasma samples obtained from patients with Alzheimer's disease and healthy individuals.