Coumarins from Leonurus japonicus and their anti-platelet aggregative activity
Yang, H.; Zhou, Q.-m.; Peng, C.; Liu, L.-s.; Xie, X.-f.; Xiong, L.; Liu, Z.-h.
Zhongguo Zhong Yao Za Zhi 39(22): 4356-4359
2014
ISSN/ISBN: 1001-5302 PMID: 25850267 Document Number: 671700
Chemical constituents of Leonurus japonicus were isolated and purified by a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, MCI, and Rp C18. Structures of the isolates were determined by spectroscopic analysis as 10 coumarins: bergapten (1), xanthotoxin (2), isopimpinellin (3), isogosferal (4), imperatorin (5), meransin hydrate(6), isomeranzin(7), murrayone(8) , auraptenol(9), and osthol(10). In addition to compound 9, the others were isolated from the genus Leonurus for the first time. In the in vitro assay, compounds 4 and 8 significantly inhibited the abnormal increase of platelet aggregation induced by ADP.