The use of fluorescent antibody technqiues to detect Theileria parva in the salivary glands of the tick Rhipicephalus appendicalatus
Kimber, C.D.; Purnell, R.E.; Sellwood, S.A.
Research in Veterinary Science 14(1): 126-127
1973
ISSN/ISBN: 0034-5288 PMID: 4196560 Document Number: 66208
Nymphs of Rhipicephalus appendiculatus Neum. were experimentally infected with Theileria parva. Developing stages of the parasite were subsequently detected in the salivary glands of the resultant adults using direct and indirect fluorescent antibody techniques. It was found that the indirect method used was more sensitive than the direct methods, probably because of difficulties in preparing a direct conjugate of sufficient activity against the tick stages of the parasite.