Growth of rinderpest and bovine virus diarrhoea viruses in Theileria parva infected lymphoblastoid cell lines
Rossiter, P.B.; Wafula, J.S.; Gumm, I.D.; Stagg, D.A.; Morzaria, S.P.; Shaw, M.
Veterinary Record 122(20): 491-492
1988
ISSN/ISBN: 0042-4900 PMID: 2844014 Document Number: 307779
T. parva parva-infected C2 lymphoblastoid line cells were infected with either rinderpest virus (the vaccine sub-strain of the Kabete 'O' strain) or bovine virus diarrhoea virus (NADL strain) before culture. In rinderpest virus cultures, specific intracytoplasmic antigen was detected within 24 to 48 hours and typical rinderpest cytopathic effects within 48 to 72 hours of infection. New extracellular virus could be detected within 48 hours with maximum titres by 72 to 96 hours after infection. Electron microscopy showed large aggregations of rinderpest virus nucleocapsid material in the cytoplasm of infected cells. The cultures were destroyed by the virus by day 7 or 8. The T. parva schizonts remained apparently healthy and no virus antigen or virus nucleocapsids were seen in the parasite. In bovine virus diarrhoea (BVD) virus cultures no antigen was detected and virus particles were not identified by electron microscopy. Increased virus infectivity was detected in culture supernatants by days 4 to 5 with maximum titres by days 7 and 8 after infection. Viral cytopathic effects were seen from day 3 and involved virtually 100% cells by day 8. Other strains of rinderpest and BVD virus have also been shown to grow in these lymphoblastoid cell lines and both viruses have grown in all of the clones of different sub-sets of B, T and null lymphoblasts that have been tested.